Ssmapkk transformations were screened on media with kanamycin ( 30mg / l ) . nineteen individual kanamycin resistant plants were obtained . t2 plants were checked for integration of foreign gene by counting ratio of the number of tolerant plants to the number of non - tolerant plants on selection medium with kanamycin ( 30mg / l ) 将ssvp和ssmapkk的全长cdna分别克隆入植物表达载体pcambia1300和prok中，导入根瘤农杆菌gv3101后，由花浸泡法进行拟南芥遗传转化，转化ssvp盐地碱蓬ssop和ssmapkk基因的克隆与功能鉴定的拟南芥在含潮霉素（ 25mg ）的ms培养基上筛选，获得t ；代转基因植株。
